Figure 1
The effect of curcumin on macrophage accumulation and gene expression in epididymal white adipose tissue (eWAT) of Western diet-induced obese mice. C57BL/6J mice were fed AIN93G diet (Control), a Western diet (WD) or a Western diet supplemented with 0.1% curcumin (WD + Cur) for 16 weeks. (a) Representative base peak, selected reaction monitoring (369.13 m/z), and tandem mass spectrometry chromatogram of curcumin in eWAT of mice fed WD + Cur for 16 weeks. (b) Representative photomicrographs showing eWAT stained with the anti-Mac2 antibody (Mac2). (c) The proportion of the Mac2-stained area and (d) eWAT area evaluated by hematoxylin–eosin staining. Values are expressed as mean ± SEM of 7–9 mice in each group. Different superscripts indicate significant differences (P < 0.05, two-sided). (e) top five canonical pathways of genes considerably upregulated or downregulated by curcumin (1), both curcumin and quercetin (2) and quercetin (3) in eWAT of diet-induced obese mice. The most considerably altered functions in the dataset were determined by the ingenuity pathway analysis. Genes substantially upregulated or downregulated by quercetin in eWAT of obese mice were previously described12 (refer Table S4 as well).
