Figure 5

Induction of Ccl9 by Cxcl16 in necrotizing acute pancreatitis. (A) Cytokine/chemokine array using pancreatic lysates from C57BL/6 mice and Cxcl16^−/− mice treated with necrotizing pancreatitis regimen as described in Fig. 2A. The relative expression of cytokines and chemokines is shown together with representative images. The data presented were obtained from WT and Cxcl16^−/− mice at 33 h, and WT on 0 h. (B) Ccl9, Vcam-1, and Ccl2 mRNA expression, relative to those of WT mice at 0 h, was assessed by qPCR in the pancreas of WT and Cxcl16^−/− mice at 33 h. (C) Ccl9 immunostaining of pancreatic frozen sections from WT mice at 0 h and 33 h, and Cxcl16^−/− mice at 33 h. *p < 0.05 Results were shown as mean ± SD.