Figure 1 | Scientific Reports

Figure 1

From: Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells

Figure 1

Experimental steps followed throughout the work. MCF-7 cells were exposed to either CoCl2- induced hypoxic or normoxic conditions. Isolation of EVs were done via ultracentrifugation. Characterization and quantification of EVs were done via NTA and EVs biosensors that are designed to capture CD-81 EVs biomarker via biotinylatd anti-CD81 antibody immobilized through streptavidin-biotin interaction on SAM modified Au SPE surface.

Back to article page