Table 1 List of reported EVs biosensor platforms.
From: Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells
Sample | Target antibody | Method | Detection range, LOD | Reference |
|---|---|---|---|---|
Ascites samples from ovarian cancer patients | CD24 and EpCAM | Nanoplasmonic detection based on transmission spectral shifts | 1 × 107 EVs/ml (3,000 EVs in 0.3 μl of sample per channel/marker) | |
Ovarian cancer patient plasma | CA-125, EpCAM, CD24 | Fluorescence CCD imaging | 7.5 × 105 particles/ml | |
hepatoma cell lines MHCC97H/L and mouse melanoma cell lines B16-F1/10 | CD9, CD41b and MET | SPR imaging | not mentioned | |
HepG2 cell line | CD63 | SPR analysis and electrochemical detection of redox label | 1 × 106 particles/ml | |
MCF-7 cell line | CD9 | Amperometric detection based on enzymatic conversion of TMB | 2 × 105 particles/ml | |
Embryonic kidney HEK293 cell lines | CD81 | EIS | 1.9 × 105 particles ml | |
MCF-7 cell line | CD63 | H2O2-mediated oxidation of TMB | 5.2 × 105 particles/ml | |
BT474 and MDA-MB-231 cell line | CD9, CD63 and HER2 | SPR | 3.3 × 107 particles/ml | |
MCF-7 cell line | CD81 | Label-free detection via DPV and EIS | 77 particles/ml for DPV and 379 particles for EIS | This work |
