Figure 2

Kinesin-1 associated proteins- UNC-16/JIP3 and UNC-76/FEZ1- regulate mitochondrial density in the anterior touch neuronal process. (A) Mitochondrial density in the ALM (anterior lateral mechanosensory) touch neuronal processes of one day adult wild type (jsIs609), miro-1(tm1966), miro-2(tm2933), miro-3(tm3150) and double mutants [miro-1(tm1966); miro-3(tm3150)], [miro-1(tm1966); miro-2(tm2933)] and [miro-3(tm3150); miro-2(tm2933)]. n ≥ 22 worms. Only significant comparisons have been marked. (B) Mitochondrial density in one day adult ALM neuronal processes of mutants in other Kinesin-1 associated proteins-unc-16(n730), unc-16(tb109), unc-16(ce451), unc-16(e109), unc-76(n2397), unc-76(e911), unc-76(n2398), unc-76(rh116), unc-14(ju56) and unc-14(e57). n ≥ 10 worms. All comparisons made to wild type (jsIs609). (C) Mitochondrial densities in one day adult wild type (jsIs609), unc-116(e2310), klc-2(km11), dhc-1(js319), unc-16(tb109), unc-76(rh116) and double mutants between Kinesin-1 adaptors and motor hypomorphs [(tb109 e2281), (tb109; km11), (js319; tb109), (e2310; rh116) and (js319; rh116)]. e2310 and e2281 are the same allele, see Table S1. n ≥ 18 worms. Black * or ns (not significant) are comparisons to wild type. Green * or ns are comparisons of adaptor single mutants and double mutants with adaptors to dhc-1 single mutant. Red * or ns are comparisons of dhc-1, adaptor single mutants and double mutants with adaptors to both unc-116 and klc-2. Blue * or ns are comparisons of motor subunit; adaptor double mutants to respective adaptor single mutants. (D) Mitochondrial density across different segments along the neuronal process in one day adult wild type (jsIs609) and mutants in Kinesin-1 associated proteins. n ≥ 18 worms. All comparisons made to wild type. Colours correspond to respective genotypes. (E) Mitochondrial density in the distal segment (last quarter) in wild type (jsIs609), unc-16 alleles, unc-76 alleles, [dhc-1(js319); unc-76(rh116)], [dhc-1(js319); unc-16(tb109)] and [dhc-1(or195); unc-16(tb109)]. n ≥ 10 worms. All comparisons made to wild type. (F) Mitochondrial densities in one day adults of wild type (jsIs609), Dynein mutants, unc-16, unc-76 and double mutants between Kinesin-1 binding proteins and Dynein mutants across different segments along the neuronal process. n ≥ 10 worms. All comparisons made between double mutants and the corresponding dynein complex single mutant. Data represented as Mean ± SEM. Statistical tests one-way ANOVA A,B,C,E) and two-way ANOVA (D,F) with Bonferroni multiple comparisons correction, ns: not significant, p value * < 0.05, ** < 0.01, *** < 0.001.