Table 1 Primers sequences used.
Gene | Primer (Forward, Reverse) | Size of product |
|---|---|---|
unc-116 | TT36, 5′CGGAAAAACACATACAATGGAGGGAGTAATCG TT37, 5′CTCGGGGTCTAATAAATCTCGAATCTTCTCG | (TT36 spans exon 2 & 3), 181 bp |
klc-2 | TT461, 5′AGGAGGCTGAGGAGTTGTACAAGC TT462, 5′GCCCATCCAGCTTGTTCGTTGG | Exon 4, 163 bp |
dhc-1 | TT46, 5′CTCTCGGACAAGTTTGGGATGC TT47, 5′CATCCCATCCTTTGATCAAACGTGTC | (TT46 spans exon 4 & 5), 186 bp |
act-3 | TT40, 5′GTAGACAATGGATCCGGAATGTGCAAGG TT41, 5′GGTACTTGAGGGTAAGGATACCTCTCTTGG | (TT41 spans exon 1 & 2) 181 bp |
miro-1 | TT408, 5′AATCTTCTACTACGCCCA TT409, 5′TTCCCAATTTTACTCCTCC | 800 bp |
miro-2 | TT193, 5′GGCTGCAGGTCAATTGACTC TT194, 5′CGAGTATCATGGACTTATCG | 1482 bp |
miro-3 | TT195, 5′TTGTACGAGTATCGTGGACT TT196, 5′GTATTCCCAGCACGACCTGA | 935 bp |
