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Figure 1

From: Super Resolution Network Analysis Defines the Molecular Architecture of Caveolae and Caveolin-1 Scaffolds

Figure 1

Computational network analysis for SMLM. (A) TIRF wide-field imaging of Cav1 and CAVIN1 and SMLM GSD imaging of Cav1 in PC3 and PC3-PTRF cells. Spot diameters from the two cell types (Experiment 1, see B) were binned (Bar: SEM; ***p < 0.001). (B) Details of 4 SMLM experiments imaged using Leica GSD microscope. (C) Methodological pipeline to discover signatures of different Cav1 domains: 3D SMLM Network Analysis. A GSD image event list is converted to a 3D point cloud. Blinks within 20 nm are merged and the 3D point cloud divided into ROIs for multi-threshold network analysis. Network measures filter the 3D point cloud to obtain clusters (blobs). Features are extracted for each blob and blob identification achieved via unsupervised learning methods.

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