Figure 3 | Scientific Reports

Figure 3

From: Suppression of mitochondrial oxygen metabolism mediated by the transcription factor HIF-1 alleviates propofol-induced cell toxicity

Figure 3

HIF-1 is activated in RCC4-EV cells under normoxic conditions. (a) 35 µg whole-cell lysates of RCC4-EV and RCC4-VHL cells exposed to either 20% O2 or 1% O2 were immunoblotted (IB) using primary antibodies raised against the indicated proteins. (b) RCC4-EV and RCC4-VHL cells were cultured with 20% O2 prior to analysis of the indicated mRNA levels using semi-quantitative qRT-PCR. Fold expression was calculated based on expression in RCC4-VHL cells incubated with 20% O2. Data presented are expressed as mean ± SD; #p < 0.05, for the indicated comparison. (c) RCC4-VHL and RCC4-EV cells were cultured for the indicated time-periods prior to cell viability evaluation by MTS assay (n = 3). (d) RCC4-VHL and RCC4-EV cells were cultured for the indicated time-periods prior to determination of the cellular ATP level. Differences between results were evaluated by t-test; #p < 0.05 for the indicated comparison.

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