Table 1 Key Resources Table.
Reagents | Identifier | Source |
|---|---|---|
Dulbecco’s modified Eagle’s medium | 11965-092 | Thermo Fisher Scientific, Waltham, MA, USA |
RPMI medium | 11875-093 | |
MitoTracker™ Green FM | M7514 | |
fetal bovine serum | SH30910 | GE Healthcare, Little Chalfont, UK |
Horseradish peroxidase-conjugated sheep anti-mouse IgG | NA931 | |
ECL prime enhanced chemiluminescence reagent | RPN2232 | |
penicillin-streptomycin | 09367-34 | Nacalai Tesque, Kyoto, Japan |
D-(−)-mannitol | 21303-32 | |
MgCl2 | 20909-42 | |
HEPES | 17557-94 | |
EGTA | 37346-05 | |
sodium pyruvate | 06977-34 | |
L-(−)-malic acid | 21030-44 | |
succinic acid disodium salt | 32405-62 | |
L(+)-ascorbic acid sodium salt | 03422-32 | |
sucrose | 196-00015 | Wako, Osaka, Japan |
KH2PO4 | 169-04245 | |
albumin from bovine serum (BSA) | 017-15141 | |
adenosine 5′-diphosphate, monopotassium salt (ADP) | 303-50751 | |
N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) | 203-12821 | |
Dimethyloxaloylglycine (DMOG) | D3695 | SIGMA, St Louis, MO, USA |
n-propyl gallate (nPG) | P3130 | |
oligomycin | O4876 | |
carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) | C2920 | |
rotenone | R8875 | |
antimycin A | A8674 | |
CellTiter 96™ AQueous One Solution Cell Proliferation Assay | G3582 | Promega, Madison, WI, USA |
Caspase-Glo™ 9 Assay Kit | G8210 | |
Apo-ONE™ Homogeneous Caspase-3/7 Assay Kit | G7792 | |
CellTiter-Glo™ luminescent cell viability assay kit | G7570 | |
RNeasy™ Mini Kit | 74104 | Qiagen, Hilden, Germany |
QuantiTect™ Reverse Transcription Kit | 205311 | |
Rotor-Gene™ SYBR Green PCR Kit | 204074 | |
anti-human HIF-1α antibody Clone 54/HIF-1α | 610959 | BD Biosciences, San Jose, CA, USA |
HIF-1β/ARNT (D28F3) XP rabbit monoclonal antibody | 5537 | Cell Signaling Technology, Danvers, MA, USA |
Complete Protease Inhibitor | 4693116001 | Roche Diagnostics, Tokyo, Japan |
Annexin V-FITC Apoptosis Detection Kit | K101 | BioVision, Milpitas, CA, USA |
Seahorse XF Plasma Membrane Permeabilizer (PMP) | 102504-100 | Agilent Technologies, Santa Clara, CA, USA |
tetramethylhydroquinone (duroquinol) | T0822 | Tokyo Chemical Industry, Tokyo, Japan |
2′,7′-dichlorofluorescin diacetate (DCFH-DA) | D399 | Molecular Probes, Eugene, OR, USA |
