Table 2 Dissociation constants (±s.e.m.) for ligands of wt hSR and Q89M-hSR calculated by fluorimetric titrations.

From: Glutamine 89 is a key residue in the allosteric modulation of human serine racemase activity by ATP

Ligand 1

Ligand 2

KD (μM)

hSR

Q89M-hSR

ATP

/

250 ± 4

154 ± 13

Glycine

4.9 ± 0.6

48 ± 5

Glycine

/

7000 ± 300

110 ± 2

ATP

470 ± 30

28 ± 3

  1. Dissociation constants for hSR were taken from18.
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