Figure 6

(A) Antifungal activity of QDs determined by broth microdilution method for Candida cells grown in the absence (Control) and presence of AIS and AIS@ZnS QDs respectively showed dose dependent percentage inhibition of growth for Candida cells. (Inset) Determination of OD600nm after 48 hrs for Candida cells grown at 30 °C in presence of AIS and AIS@ZnS QDs respectively. (B) Cellular uptake of AIS and AIS@ZnS QDs is estimated by relative fluorescence (Rf) intensity values and Y axis depict the mean Rf values ± the standard deviation (SD) of three sets of independent experiments. Rf Intensity was calculated by subtracting the fluorescence intensity for cells incubated without QDs (blank) from fluorescence intensity for cells incubated with AIS and AIS@ZnS QDs respectively. (C) Confocal images showing cellular uptake of QDs after incubation for 4 and 6 hours. The blue fluorescence inside the fungal cells showed the internalization of the QDs.