Figure 8

Confocal images showing green fluorescence for time dependent intracellular ROS produced in fungal cells in response to QDs. Endogenous ROS was assessed in Candida cells grown in the absence (Control) and presence of 31.25 µg/ml (sub-inhibitory) and 62.5 µg/ml (MIC₉₀) of AIS and also in presence of 62.5 µg/ml (sub-inhibitory) and 125 µg/ml (MIC₉₀) of AIS@ZnS respectively at different time intervals (4 hr, 8 hr, 16 hr and 24 hr). Each left panel of different time intervals depict the fluorescence image (higher fluorescence indicates higher ROS production) taken in a Confocal microscope and each right panel for different time intervals represents the DIC image at magnification 100X. The obtained results were compared with Control (Untreated) and positive control (cells treated with 10 mM hydrogen peroxide, H₂O₂).