Figure 4
Validations of SBSN in epidermal differentiation. (a) Heatmap of the expression levels between Sbsn knockdown mice and controls. Expression levels are shown for genes differentially expressed (under |log2-fold-change| >0.5 and q-value < 0.05) upon Sbsn knockdown. Red and white colors indicate high and low expression levels (arc-sine hyperbolic transformed normalized counts by DESeq and scaled by standard deviations) for 326 up-regulated genes, respectively. Blue and white colors indicate high and low expression levels for 161 down-regulated genes, respectively. (b) Expression values of SBSN normalized by 18 S rRNA in differentiated keratinocytes upon IL-4 treatment. To evaluate the gene expression changes of SBSN during keratinocyte differentiation upon IL-4 treatment, an RT-PCR experiment was performed with nine differentiated cells with and without IL-4 treatments (three replicates per condition). The expression values of SBSN were normalized by the expression levels of 18 S rRNA. The boxplot shows a significant decrease of SBSN expression at two IL doses (5 ng/ml and 50 ng/ml) (*p-value \( < 0.05\)). (c) Expression values of full-length SBSN transcript (v1) in AD skins. To evaluate the expression changes of SBSN in AD skins, expression values were measured in AD skins for the SBSN transcripts via RT-PCR. The expression levels were normalized by the expression levels of G6PD. The full-length SBSN transcript showed significantly decreased expression levels in AD lesional skins compared to AD nonlesional and control skins (***p-value \( < 0.001\), **p-value \( < 0.01\), *p-value \( < 0.05\)).
