Figure 2
Expression pattern of NtLTP4. (a) Tissue-specific expressions of NtLTP4 in roots, leaves and stems were quantified by qRT-PCR. (b) Total RNA extracted from the whole plants of 14-day-old N. tabacum without treatment and used for qRT-PCR. (c–i) Total RNA extracted from the whole plants of 14-day-old N. tabacum exposed to 200 mM NaCl, 200 mM mannitol, 100 μM ABA, wounding, 5 μM SA, 500 μM MeJA and R. solanacearum were used for qRT-PCR, respectively. Lanes represented the various durations of treatments, the internal control gene was Actin. Experiment was repeated three times. Error bars indicated SEM. One-way ANOVA (Duncan’s multiple range test) was performed, and statistical significant differences were indicated by different lowercase letters (P < 0.01).
