Figure 7

Overexpression of NtLTP4 in transgenic plants decreased the ROS production and oxidative damage under salt and drought treatments. (a) Photographs of NBT staining and O2− concentrations measured by kit in WT and OE plants after drought and salt treatments. Bar = 2 cm. (b) Photographs of DAB staining and H2O2 concentrations measured by kit in WT and OE plants after drought and salt treatments. Bar = 2 cm. (c) MDA contents in WT and OE plants after salt and drought treatments. (d) SOD, POD and CAT activities measured by corresponding kit, respectively. (e) The expression levels of ROS-scavenging or ROS-producing genes APX, CAT, GST, SOD, RbohA and RbohB in WT and OE lines. Data as mean values of three biological repeats. Error bars indicated SEM. One-way ANOVA (Duncan′s multiple range test) was performed, and statistical significant differences were indicated by different lowercase letters (P < 0.01).