Figure 8 | Scientific Reports

Figure 8

From: NtLTP4, a lipid transfer protein that enhances salt and drought stresses tolerance in Nicotiana tabacum

Figure 8

Interaction of NtLTP4 with WIPK. (a) Detection of pGBKT7-NtLTP4-Myc bait fusion protein via Western Blot. Lane 1: pGBKT7 empty insert (negative control), lane 2: NtLTP4-pGBKT7-Myc. (b) Yeast two-hybrid screening assay was conducted in yeast. pGBKT7-p53 and pGADT7-T were used as positive control, and pGBKT7-pLam and pGADT7-T were used as negative control. (c) NtLTP4 interacted with WIPK by yeast-two-hybrid. Bait fusion vector and prey fusion vector pGBKT7-NtLTP4/pGADT7-WIPK and pGBKT7-WIPK/pGADT7-NtLTP4 were transformed into the yeast strain Gold Yeast. (d) NtLTP4 interacted with WIPK by LCI assay. Bar = 2 cm, (e) The interaction between NtLTP4 and WIPK was tested via co-immunoprecipitation (Co-IP) assay of transiently overexpressed WIPK-HA and NtLTP4-GFP in N. benthamiana leaves. N. benthamiana leaves that co-expressed GFP and WIPK-HA were used as negative controls.

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