Figure 1
From: Leptin Promotes Allergic Airway Inflammation through Targeting the Unfolded Protein Response Pathway
Leptin induces expression of the UPR factor XBP1s. (A) Immune blot of XBP1s expression in in vitro generated TH2 cells with or without treatment with leptin. (B) ELISA of leptin expression in sera of HFD and ND mice. (C) Flow cytometry of ObR expression on LLN ILC2, TH2 and TH1 cells from asthmatic ND and HFD mice. (D) Western blot of XBP1s expression in splenic CD4+ T cells from ND and HFD mice. (A right, D right) Quantification of XBP1s abundance was relative to β-Actin. (A right, B,D right) Values are means and SD [n = 3 (A right) or 4–6 per group (B,D right)]. Student’s t-test *p ≤ 0.05 and **p ≤ 0.005. Data represent 2 (B,C,D) or 3 (A) experiments.
