Figure 3
From: Leptin Promotes Allergic Airway Inflammation through Targeting the Unfolded Protein Response Pathway
HFD leads to increased type 2 lymphocyte responses. (A,B) Intracellular stain of TH2 (LIN+CD4+IL-13+), ILC2s (LIN−CD4−IL-13+) (A) and TH1 cells (B) in LLN cells from asthmatic ND and HFD mice. (C,D) Quantification of TH2, ILC2 and TH1 cells in asthmatic LLNs (C) and non-asthmatic LLNs (D). (E) ELISA of cytokine expression by asthmatic LLN cells after ex vivo recall with Ova at indicated concentrations. (F) Intracellular stain of Ki67 in LLN TH2 cells, ILC2s and TH1 cells in asthmatic LLN cells. (C,D,E,F bottom) Values are means and SD. Student’s t-test, *p ≤ 0.05 and **p ≤ 0.005. Data represent 2 experiments (n = 4–6 per group).
