Figure 4
From: Leptin Promotes Allergic Airway Inflammation through Targeting the Unfolded Protein Response Pathway
XBP1s mediates leptin-induced TH2 cytokine production. (A) Western blot of XBP1s abundances with Tubulin as a loading control in in vitro differentiated TH2 cells following transfection of siXbp1 or scramble siRNA (Sc) for indicated times in the presence of leptin for 40 h. (B) RT-qPCR of mRNA expression in TH2 cells treated as (A). mRNA abundances were normalized to an internal housekeeping gene Actb. (C) ELISA of cytokine expression in TH2 cells treated as (A). (A right, B,C) Values are means and SD (n = 3–4 biological replicates per group). Student’s t-test, *p ≤ 0.05, and **p ≤ 0.005. Data represent 2 (B,C) or 3 experiments (A).
