Figure 2

(a) Temporal-expression profiles of Slc9a3r1 mRNA in the livers of wild-type and Per2^m/m mice maintained under a 12-h light/dark cycle (left) and constant dark condition (right). Each value represents the mean with s.e.m. of results for 4–6 mice. There were significant time-dependent variations in Slc9a3r1 mRNA levels in wild-type mice (F_5,30 = 5.829; P < 0.001; ANOVA). **P < 0.01; *P < 0.05, significantly different from wild-type mice at the corresponding time points (F_11,92 = 5.764; P < 0.001; ANOVA with Tukey-Kramer post hoc test). ^##P < 0.01, significant difference between the two groups (F_3,12 = 37.113, P < 0.001; ANOVA with Tukey-Kramer post hoc test). (b) Temporal-expression profiles of NHERF1 protein in the hepatic plasma membrane of wild-type and Per2^m/m mice maintained under a 12-h light/dark cycle (left) and constant dark conditions (right). Each value represents the mean with s.e.m. for data from of 4–10 mice. There were significant time-dependent variations in the protein levels of NHERF1 (F_5,54 = 28.402; P < 0.001; ANOVA). *P < 0.05, significantly different from wild-type mice at the corresponding time points (F_11,119 = 5.618; P < 0.001; ANOVA with Tukey-Kramer post hoc test). ^##P < 0.01, significant difference between the two groups (F_3,12 = 37.113, P < 0.001; ANOVA with Tukey-Kramer post hoc test). For both panels, the mean peak values in wild-type mice were set at 100. Full-size images of immunoblotting are presented in Supplementary Fig. 12.