Figure 2 | Scientific Reports

Figure 2

From: Super-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device

Figure 2

Reconstruction of the 3D RI tomogram of a sample. (a) Structured illumination generated by a DMD is the superposition of two plane waves with a controlled wave vector and phase shift Δϕ. (b) Time-multiplexed patterns displayed on the DMD and (c) the corresponding holograms of a sample. Insets: magnified image (upper) and corresponding Fourier spectrum in the spatial frequency k space (lower). Except for sample illumination at a normal angle, a pair of patterns projects two plane waves with different phase shifts onto a sample to decompose diffracted optical fields with respect to individual plane wave components. (d) Phase images of the retrieved optical fields corresponding to the plane wave components with various incident angles. Inset: corresponding Fourier spectrum. (e) A mapped object function in 3D Fourier space using the principle of ODT.

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