Figure 3 | Scientific Reports

Figure 3

From: Super-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device

Figure 3

Reconstruction of the 3D fluorescence image of a sample. (a) Epifluorescence microscopy geometry for structured illumination excitation of a sample and recording its fluorescence emission. A DMD controls both the phase shift and the illumination angles of three plane waves that result in the structured excitation pattern on a sample. (b) Time-multiplexed patterns displayed on the DMD (inset: corresponding Fourier spectrum) and (c) corresponding fluorescence images of a sample (inset: magnified images). (d) and (e) Illustrations in Fourier space for describing the SIM reconstruction from (d) measured fluorescence images to (e) reconstructed SIM images. (f) Reconstructed SIM and wide-field fluorescence (WF) images of a cluster of red fluorescent microspheres.

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