Figure 4 | Scientific Reports

Figure 4

From: Super-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device

Figure 4

3D RI distribution and 3D fluorescence image of a cluster of red fluorescent PS beads. (a) and (b) Cross-sectional slices of reconstructed 3D RI and (c–f) 3D fluorescence distributions in x-y, x-z, and y-z planes of a cluster of red fluorescent 500-nm-diameter PS beads. The slices in each column were reconstructed using (a,b) ODT, (c) and (d) SIM, and (e,f) WF. The slices in (b,d,f) are magnified images of the area in the dashed boxes in (a,c), and (e,g) and (h) Cross-sectional slices of the 3D Fourier spectrum of 3D fluorescence distributions reconstructed using WF and SIM, respectively. Illustrations on the right-hand side of (g,h) are representations of the theoretical transfer function of WF and SIM.

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