Figure 6
SORT1 deficiency suppressed LXR-mediated transcription in human Caco-2, HepG2, and Hek293 cells. (a) Sortilin protein reduction confirmation in Caco-2, HepG2, and Hek293 cells following SORT1 RNA interference (siSort1) or scrambled control (Scr), with or without 24-hour 1 μM T0901317 (T090) LXR agonist treatment; (n = 3; representative western blots shown). GAPDH loading controls were run on the same blot, and the membrane was cut prior to incubating with primary antibody. Full-length blots are presented in Supplemental Fig. S4c. (b) Caco-2, (c) HepG2, and (d) Hek293 cells SORT1, LXRα/β (NR1H3, NR1H2), and KLF4 mRNA levels following SORT1 RNA interference, with or without 24-hour 1 μM T090 treatment; (n = 3–6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. Scr control, analyzed by ANOVA with Tukey’s multiple comparisons test; values are presented as mean ± SEM.
