Table 1 Selected candidate reference genes, their corresponding product name, primer sequences (annealing temperature of 60 °C), amplicon size in base pairs (bp), their respective PCR amplification efficiencies and the mean CT values(±standard deviation) assessed in K. pneumoniae cells submitted to various experimental conditions and at different phases of growth.
Genes | Product Name | Forward and Reverse primer sequences (5′ > 3′) | Amplicon size | R2 | E (%) | Mean CT ± SD |
|---|---|---|---|---|---|---|
proC | Pyrroline-5-carboxylate reductase | GATTGCCGATATCGTCTTCG GAGACCACCAGCGACTCTTT | 99 bp | 0.989 | 83.4 | 24.91 ± 0.62 |
glnA | Glutamine synthetase | GAAGGCGGTAACAAAGGTCA TACACATGGTGGAACGGATG | 97 bp | 0.988 | 98.2 | 23.79 ± 1.19 |
gyrA | DNA gyrase subunit A | GTGACCCGTCGTACGATTTT GATAATCGGGTCGATGTTGG | 99 bp | 0.987 | 96.6 | 24.45 ± 1.10 |
recA | Recombinase A | TTAAACAGGCCGAATTCCAG CCGCTTTCTCAATCAGCTTC | 99 bp | 0.989 | 92.1 | 22.40 ± 0.61 |
rpoD | RNA polymerase sigma factor RpoD | TCCGGTGCATATGATTGAGA ATACGCTCAGCCAGCTCTTC | 105 bp | 0.989 | 87.5 | 25.05 ± 0.80 |
rho | Transcription termination factor Rho | AACTACGACAAGCCGGAAAA ACCGTTACCACGCTCCATAC | 99 bp | 0.998 | 92.4 | 23.92 ± 0.90 |
rpoC | DNA-directed RNA polymerase subunit beta’ | TATTCTGGTTCCACGCAACA GGATACAACGGAACGCACTT | 97 bp | 0.969 | 91.2 | 23.28 ± 0.88 |
rrsH | 16S ribosomal RNA | GACGATCCCTAGCTGGTCTG GTGCAATATTCCCCACTGCT | 95 bp | 0.957 | 94.2 | 10.60 ± 0.55 |
aat | Leucyl/phenylalanyl-tRNA-protein transferase | CTGGATAACCAGCAGTATCGTTC GTACATTCCACCTACCAGCGTATT | 106 bp | 0.994 | 84.1 | 38.83 ± 1.55 |
ffh | Signal recognition particle protein | GCTAAGCCGGAAATCATCAA ATGTCGTCGAACTGCTTGAG | 104 bp | 0.986 | 97.2 | 23.93 ± 1.39 |
trpS | Tryptophanyl-tRNA synthetase II | GCCACTGTAAGGCGCTACTC GCCGATAACGTCAGCGTATT | 100 bp | 0.992 | 87.1 | 29.14 ± 1.50 |
