Figure 6
From: Substance P modulates bone remodeling properties of murine osteoblasts and osteoclasts
Osteoclast- and osteoblast-specific marker gene expression. Comparison of RANK (A), MMP-9 (B), CTSK (cathepsin K; C) and NFATC1 (D) gene expression in Tac1−/− to WT BMM/osteoclast cultures after 5 days of M-CSF and RANKL mediated differentiation. N = 5–11. Comparison of TNFRSF11B (OPG Day 14; E), TNFSF11 (RANKL Day 21; F), BGLAP (osteocalcin day 21; G), RUNX2 (Day 21; H) gene expression in Tac1−/− to WT osteoblasts after 14 (E) and 21 (F–H) days in osteogenic medium. N = 8–16. Results of RNA isolated from Tac1−/− cells were calibrated to RNA isolated from WT cells ( = x-axes/0-line). Ø = no stimulation.
