Figure 3 | Scientific Reports

Figure 3

From: A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation

Figure 3

Overview over the steps required for forming proteo-GUVs. (a) Flowchart representing the workflow for preparation of protein-containing GUVs, starting from the preparation of SUVs. (b) Snapshots of the electroformation of SNARE-GUVs on a Pt wire. Membranes were labelled with the lipophilic dye DiO for fluorescent visualization. GUVs are indicated with red arrows. Scale bar 50 µm. See also Supplementary Video 1.

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