Figure 3
Inhibition of miR-10a-3p reduced osteogenic phenotype of OPLL cells. The effect of miRNAs inhibition on osteogenic properties of OPLL ligament cells were analyzed using Alizarin Red staining (A) or alkaline phosphatase staining (B) at 12 days after osteo-induction. The colorimetric quantification were shown in the right panels. *P < 0.05, **P < 0.01, n = 3, t-test. (C) Real-time PCR analysis of ossification related genes in each treatment group (n = 3) of induced OPLL ligament cells. Blank represents osteo-induced cells with no treatment, while NC represents overexpression of a scramble Antagomir served as negative control. *P < 0.05, **P < 0.01, t-test. (D) Western blot analysis of ossification related gene products in each treatment group (n = 2), uncropped images were shown in the Supplementary Fig. 5. The error bars represents ± S.D.
