Figure 2
Comparative HPLC analysis of the pigment content of RCC1774 and the control Chl d-containing strain Acaryochloris sp. HCIR111A. (a,b) Chromatograms using the method described by Zapata and co-workers38. Pigments shared by the two strains (i.e. same retention time and absorption spectrum) are indicated by numbers: 1, Mg-2,4-divinyl pheoporphyrin a5; 2, possible caloxanthin (monohydroxy-zeaxanthin); 3, zeaxanthin; 4, 5 & 7, unidentified carotenoids; 6, Chl a; 8 β,ε-carotene (a.k.a. α-carotene). RCC1774-specific pigments: a, Chlide a; b, possible nostoxanthin; c & d, unidentified carotenoids; e, Chl b. HCIR111A-specific pigments: a′ & b′, Chlide d-like pigments; c′ & d′, unidentified carotenoids; e′, Chl d. (c,d) On-line absorption spectra of Chl b (RCC1774) and Chl d (HCIR111A); for other peaks, see Supplementary Fig. S2.
