Table 3 MAT results from the 46 patients with laboratory-confirmed leptospirosis.

From: Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease

Kinetics of Leptospira infection

Serovar

Species

Profiles

Conventional nested PCR (N = 46)

MAT

qRT-PCR-HRM

C

1

Positive (co-agglutination – highest titre 1:1280 against Arb A and Cyn)

L. borgpetersenii

1

Positive (Arb A 1:160)

L. borgpetersenii

1

Positive (co-agglutination – highest titre 1:320 against Arb A 1:320)

L. interrogans

1

NC

L. borgpetersenii

6

Negative

L. interrogans

B

1

NC

L. interrogans

1

NC

L. interrogans

16

Negative

L. interrogans

10

Negative

L. borgpetersenii

A

3

Negative

L. interrogans

5

Negative

L. borgpetersenii

  1. Abbreviations: Arb A [serovar Arborea (Azorean isolate) serogroup Ballum]; Cyn [Cynopteri (reference serovar) serogroup Cynopteri]; NC, not conclusive (specific reactivity below the cut-off of 1:160 adopted in the Azorean endemic region).
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