Table 5 Comparison between qRT-PCR-HRM (SYBR) and qRT-PCR TaqMan for diagnosis of leptospirosis in a clinical setting.

From: Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease

Patients (N) suspected of Leptospirosis

Clinical samples

Leptospira

Diagnostic accuracy

Country

(geographic area)

Paper (year)

Serum (N)

Urine (N)

Detection

method

Molecular target

Nr. Positives (%)

Sensitivity

Specificity

Positive

predictive value (PPV)

Negative

predictive value (NPV)

202

202*

202*

qRT-PCR-HRM

lfb1 and secy

46 (22.7)

100 (90–100)

100 (97–100)

100 (90–100)

100 (97–100)

Portugal

(Azores)

Current study

295

253 plasma

121

qRT-PCR-HRM

MAT

lipL32

Ig

15 (5.0)

60

100

100

100

Czech Republic

Cermakova Z. et al.22

235

235

0

qRT-PCR-HRM

MAT

lipL32

Ig

26 (11.0)

30

100

100

100

Uruguay

González S. et al.19

133

133

0

qRT-PCR-HRM

secy

26 (19.5)

100 (70–100)

100 (93–100)

NR

NR

Netherlands

Ahmed A. et al.17

266

133

blood

0

qRT-PCR TaqMan

qRT-PCR TaqMan

MAT

Culture

rrs PCR

lipL32

Ig

Leptospira

74 (55.6)

57 (42.8)

115 (86.4)

39 (29.3)

56 (47–64)

43 (34–52)

86 (79–92)

29 (22–38)

90 (83–94)

93 (88–97)

100

100

NR

NR

Thailand

Thaipadungpanit J. et al.18

787

785

644

qRT-PCR TaqMan

qPCR

MAT

Culture

rrs

16S rRNA/LipL32

Ig

Leptospira

76 (9.7)

20 (2.5)

30 (3.8)

4 (0.5)

50 (29.6–77.8)

53.9 (33.3–81.8)

15.8 (6.3–29.4)

25.0 (13.3–44.4)

99.2 (99–99.5)

99.6 (99.2–100)

96.5 (96.2–96.9)

100

57.1 (42.9–71.4)

75 (50–100)

10 (3.3–20)

100

99 (97.3–99.7)

99.1 (97.6–99.7)

98.3 (96.7–98.9)

98.5 (96.7–99.4)

Laos

Woods K. et al.21

150

150

0

qRT-PCR TaqMan

lipL32

127 (84.6)

29.1 (21.6–38.0)

99

NR

NR

Brazil (Salvador and Curitiba)

Riediger I. N. et al.33

  1. *Analysed in duplicate; NR, not reported.
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