Figure 2
From: MiR-99b-5p and miR-203a-3p Function as Tumor Suppressors by Targeting IGF-1R in Gastric Cancer
Ectopic expression of miR-99b-5p and miR-203a-3p in GC cells affects cell viability, colony formation, cell cycle and apoptosis in vitro. (A) Enforced expression of miR-99b-5p and miR-203a-3p was detected by qRT-PCR after transfected with pre-miR-99b and pre-miR-203a vectors in MKN-45/SGC-7901 cells. (B) GC cells expressing miR-99b-5p and miR-203a-3p show a decreased viability compared to cells expressing the negative miRNA control. (C) The percentage of colonies shows a significantly decrease in cells expressing miR-99b-5p/203a-3p. (D) Effects of miR-99b-5p/203a-3p overexpression on the cell cycle progression of MKN-45/ SGC-7901 cells measured by flow cytometric analysis. The expression of Cyclin D1 and CDK4/6 was analyzed by western blot. (E) Over-expression of miR-99b-5p/203a-3p significantly promotes cell death in GC cells. (F) The expression of Bax and Bcl2 was analyzed by western blot (*P < 0.05, **P < 0.01, Student’s t test or Mann-Whitney test).
