Figure 3
From: MiR-99b-5p and miR-203a-3p Function as Tumor Suppressors by Targeting IGF-1R in Gastric Cancer
Inhibiting miR-99b-5p/203a-3p contributes to gastric cell growth. (A) qRT-PCR analysis of MKN-45/SGC-7901 cells after transfection with anti-miR-99b-5p/203a-3p or a negative control. (B,C) MTT assay and colony formation were used to assay the effects of anti-miR-99b-5p/203a-3p in MKN-45/SGC-7901 cells. (D,E) Cell cycle and apoptosis were determined in MKN45/SGC-7901 cells transfected with anti-miR-99b-5p/203a-3p or a negative control. The expression of Cyclin D1 and CDK4/6 was analyzed by western blot. (F) The expression of Bax and Bcl2 was analyzed by western blot (*P < 0.05, **P < 0.01, Student’s t test).
