Table 1 Primers for reverse transcription-polymerase chain reaction (RT-PCR).
From: Gallic acid improves cardiac dysfunction and fibrosis in pressure overload-induced heart failure
Gene | Primer sequence (5′ to 3′) |
|---|---|
GAPDH (mouse) | F: GCATGGCCTTCCGTGTTCCT R: CCCTGTTGCTGTAGCCGTATTCAT |
Collagen type I (mouse) | F: GAGCGGAGAGTACTGGATCG R: GCTTCTTTTCCTTGGGGTTC |
SMA (mouse) | F: ACTGGGACGACATGGAAAAG R: AGAGGCATAGAGGGACAGCA |
Fibronectin (mouse) | F: GATGCACCGATTGTCAACAG R: TGATCAGCATGGACCACTTC |
CTGF (mouse) | F: CAAAGCAGCTGCAAATACCA R: GGCCAAATGTGTCTTCCAGT |
P8 (mouse) | F: CCTTCCCAGCAACCTCTAAA R: GAACTTGGTCAGCAGCTTCC |
MMP2 (mouse) | F: ATGCCATCCCTGATAACCTG R: TGTGCAGCGATGAAGATGAT |
MMP9 (mouse) | F: GAAGGCAAACCCTGTGTGTT R: AGGAAGACGAAGGGGAAGAC |
MMP13 (mouse) | F: CCAGAACTTCCCAACCATGT R: GTCTTCCCCGTGTTCTCAAA |
β-MHC (mouse) | F: GCATTCTCCTGCTGTTTCCT R: CCCAAATGCAGCCATCTC |
α-MHC (mouse) | F: ATAAAGGGGCTGGAGCACTG R: GCCTCTAGGCGTTCCTTCTC |
GAPDH (rat) | F: AACCCATCACCATCTTCCAGGAGC R: ATGGACTGTGGTCATGAGCCCTTC |
Collagen type I (rat) | F: ACCCCAAGGAGAAGAAGCAT R: AGGTTGCCAGTCTGTTGGTC |
SMA (rat) | F: GACACCAGGGAGTGATGGTT R: GTTAGCAAGGTCGGATGCTC |
Fibronectin (rat) | F: GAGCGGAGAGTACTGGATCG R: GCTTCTTTTCCTTGGGGTTC |
CTGF (rat) | F: CCTTGGTGCTCCTCCTCTG R: AGTCGCAGAAGAGACCCTTG |
