Figure 2 | Scientific Reports

Figure 2

From: Inhibitory effect of SLIT2 on granulosa cell proliferation mediated by the CDC42-PAKs-ERK1/2 MAPK pathway in the prehierarchical follicles of the chicken ovary

Figure 2

Effects of the SLIT2 knockdown on the expression of the ROBO1 and ROBO2 genes. The granulosa cells were transfected with the SLIT2-specific siRNA, scrambled siRNA (negative control) or no siRNA (blank control). (A) The expression of the SLIT2 gene in the GCs with or without the interference of the specific siRNA was determined by qRT-PCR. The mRNA expression was normalized to that of the 18S rRNA gene; the values of the bar graphs represent the mean ± SEM of 10 hens (n = 10) from a representative experiment. (B) The expression levels of the SLIT2 protein in the GCs with or without the siRNA interference were detected by western blotting. β-actin was used as a loading control. (C) The influence of the SLIT2 knockdown on the ROBO1 and ROBO2 mRNA abundance in the granulosa cells. (D) The effects of the SLIT2 knockdown on the protein levels of ROBO1 and ROBO2. All blots were cropped, and the gels were run under the same experimental conditions. For each group, the superscript symbol above the bar indicates that the difference was significant compared to the control group **P < 0.01, *P < 0.05.

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