Table 4 Primer Sequence and the Reaction Condition for Genotyping TNFR2 Polymorphisms.

From: The association between TNFR gene polymorphisms and the risk of Hepatitis B Virus-Related Liver Diseases in Chinese population

SNP

Primer Sequence

Annealing temperature

Product size (bp)

Restriction enzyme

rs1061622

Forward:5′-TCCTCCAGCTGTAACGTGG-3′

63 °C

TT:246 bp, 45 bp

Hin1II

Reverse:5′-GACAGGCAGACAGAAGGAGT-3′

 

GG:291 bp

 
  

TG:291 bp, 246 bp, 45 bp

 

rs1061624

Forward:5′-TGGGCCAAGTTCCTCTAGTG-3′

58 °C

GG:71 bp, 106 bp

MspA1I

Reverse:5′-CAGGTCACAGAGAGTCAGGG-3′

 

AA:177 bp

 
  

GA:177 bp, 71 bp, 106 bp

 
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