Figure 8

Data process for SPROT reconstruction. (a) The post- and pre-injection projection subtraction approach. The calibrated post- and pre-injection optical images were registered and subtracted. This process suppresses the artifact otherwise caused by the high intrinsic absorption from the mouse abdomen. The surface fluence data was simulated based on a homogeneous 3D mesh generated from the XCT, and then added to the subtraction image to form the combined surface fluence data. The combined data was used for SPROT reconstruction. (b) The array of detector locations used in SPROT reconstruction. Each cross represents a detector point. (c) Comparison of the simulated and measured surface fluence data demonstrates a similar light distribution pattern. The arrows point to the high intensity regions which appeared in the same location. The normalization factor was calculated as the ratio of simulation to measurement data. The redline outline indicates the region of useful detector data.