Figure 6 | Scientific Reports

Figure 6

From: ERK1/2 and p38 regulate inter-individual variability in ozone-mediated IL-8 gene expression in primary human bronchial epithelial cells

Figure 6

Comparison of p65 activation in high and low-responding cultures. (A) Using the same panel of donors as Fig. 4, we examined the p65 phosphorylation at S536, an indicator of canonical NFκB activation. Primary cells were stimulated with 10 ng/mL TNFα for 20 minutes as a positive control. Representative donors shown are the same donors depicted in Fig. 4. Both sets of blots were run on the same gel, immunoblotted, and imaged at the same time. (B) Median and interquartile range of ozone-induced phosphorylation changes across 6 high and 6 low responders (n = 12) as calculated by densitometric comparison to filtered air controls. No significant difference was found between these two groups (p = 0.07). (C) Linear regression showing the correlation between ozone-associated changes in p65 phosphorylation and IL-8 mRNA expression.

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