Figure 5
Soluble CD146 decreases senescence in ECFC isolated from peripheral blood of patients. (A) Effect of 48 hours of treatment with 50 ng/ml sCD146 on the senescence of pb-ECFC and comparison with cb-ECFC. Representative pictures are shown. The results are the mean values +/− SEM of 4 different experiments. (B) The ratio of senescent cells to the total number of cells is given in pb-ECFC and pb-ECFC treated for 48 h with 50 ng/ml sCD146 as a function of the % of confluence of the cells (left panel) and of the number of passages of the cells (right panel). The results are the mean values +/− SEM of 9–11 independent experiments. (C) Effect of 48 h of treatment with 50 ng/ml sCD146 on the protein expression of p21WAF, p16INK4a, p53 and SIRT1 in pb-ECFC at passages 2–3 and passages 4–5. The same actin was used for p53 and sirt1 because the western-blot membrane was cut in three parts for revealing with the three antibodies. Representative pictures are shown, and mean values +/− SE of 4 experiments are given. *P < 0.05, **P < 0.01, experimental vs. Control. To improve the clarity and conciseness of the figure, cropped gels corresponding to the band of interest are shown. β-actin was visualized and analysed on the same gel. Quantitative comparisons between samples were performed on samples migrated on the same gels.
