Figure 6

Mature miR-21 coordinates soluble CD146 effects. (A) Effect of 48 hours of treatment with 50 ng/ml of soluble CD146 on mature miR-21 expression in pb-ECFC. Mean values +/− SE of 4 experiments are given. (B) Effect of anti-miR-21 on the sCD146-induced increase in pb-ECFC proliferation (48 hours of treatment with 50 ng/ml sCD146). Mean values +/− SE of 4 experiments are given. (C) Effect of anti-miR-21 on the sCD146-induced increase in the number of sprouts, branch points and cumulative sprout length in spheroid assays performed with pb-ECFC (48 hours of treatment with 50 ng/ml sCD146). For each experiment, sprouts from 20 spheroids were counted. Mean values +/− SE of 6 independent experiments are given. (D) Effect of anti-miR-21 on the sCD146-induced increase in Oct-4 (48 hours of treatment with 50 ng/ml sCD146). Representative pictures are shown and mean values +/− SE of 4 independent experiments are given. (E) Effect of anti-miR-21 on the sCD146-induced modulation of PECAM-1 and α-SMA in pb-ECFC (48 hours of treatment with 50 ng/ml sCD146). Representative pictures are shown and mean values +/− SE of 5 independent experiments are given. (F) Effect of anti-miR-21 on the sCD146-induced decrease in PTEN in pb-ECFC (48 hours of treatment with 50 ng/ml sCD146). Representative pictures are shown and mean values +/− SE of 4 independent experiments are given. (G) Effect of anti-miR-21 on the sCD146-induced decrease in the number of senescent pb-ECFC (48 hours of treatment with 50 ng/ml sCD146). Representative pictures are shown and mean values +/− SE of 4 independent experiments are given. *^,**^,***P < 0.05, P < 0.01, P < 0.001, experimental vs. Control.