Figure 2 | Scientific Reports

Figure 2

From: Transient AID expression for in situ mutagenesis with improved cellular fitness

Figure 2

Stable AID expression induces a spike of RFP fluorescence loss. 293FT/RFP1 cells were stably transduced with AID mutants and then 105 cells were analyzed for somatic hypermutation by RFP fluorescence loss using a flow cytometer (A) 10 days after stable expression or (B) periodically over one month. The results show the mean values of three replicates ± S.D. Significant differences between RFP loss after expression of S38A or m7.3 mutants compared to AID-WT are indicated; **P ≤ 0.01.

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