Figure 6
From: Transient AID expression for in situ mutagenesis with improved cellular fitness
Transient expression of AID reduces its adverse effect on cell growth. Growth curve of HEK293FT cells (A) virally transduced with AID mutants or (B) transiently transfected with AID expressing plasmids. Results show mean values ± SD (n = 3). (C) Western blots show AID expression in cell extracts from 3T3 and 3T3 TetOn-AID cells after 4 days of culturing the cells with or without 0.5 µg/mL doxycycline; tubulin was used as loading control. (D) AID induction as confirmed by bicistronic GFP expression in 105 cells of each 3T3 and 3T3 TetOn-AID cells after 4 days of culturing the cells with or without 0.5 µg/mL doxycycline. (E) Growth curve of 3T3 and 3T3 TetOn-AID cells with or without 0.5 µg/mL for 6 days. Results show mean values ± SD (n = 3). Significant differences between growth curves are indicated; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. ns, not significant.
