Figure 2 | Scientific Reports

Figure 2

From: Chalcomoracin is a potent anticancer agent acting through triggering Oxidative stress via a mitophagy- and paraptosis-dependent mechanism

Figure 2

CMR induced paraptosis in PC-3 and MDA-MB-231 cell lines, but not LNCaP cell line and apoptosis in MCF-10A. (a) The effect of CMR on apoptosis, autophagy, ER-stress, and paraptosis-related proteins expression in PC-3, MDA-MB-231, LNCaP and MCF-10A cells. Cells were treated with varying concentrations of CMR for 48 h. Cropped blots were displayed, and original images were included in Figs S17–S20. (b) Immunocytochemical analysis of the effect of CMR on cellular distribution of GFP-tagged LC3 ectopically expressed in PC-3 and MDA-MB-231 cells. Cells were transfected with GFP-tagged LC3 plasmids for 24 h, treated with 6 μM CMR for 48 h, and then stained. Blue: DAPI. Red: mitotracker (0.5 μM). Green: GFP-LC3. Representative image of 3 independent experiments is shown.

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