Figure 4 | Scientific Reports

Figure 4

From: Systematic analysis of biological roles of charged amino acid residues located throughout the structured inner wall of a virus capsid

Figure 4

Intrinsic Trp fluorescence analysis of a heat-induced conformational rearrangement of the MVM capsid. The fraction of VP2-only capsids in the final state conformation is represented as a function of temperature. Circles, non-mutated wt control; red triangles, E146A mutant; blue inverted triangles, E264A mutant. The intrinsic Trp fluorescence of the D263A mutant as a function of temperature was determined as a part of a previous study with a different goal66. The T m for this transition in the wt capsid varied within <1 °C in 4 independent experiments carried out for this study.

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