Table 2 Frequency of immunohistochemical detection of PG and TF in each location and PCR results.
Diagnosis | Number (%) of samples with reactivity | Number (%) of samples with intracellular reactivity located in | Number (%) of positive samples by PCR | |||
|---|---|---|---|---|---|---|
Bacterial species detected by IHC and PCR | Extracellular | Intracellular | Stromal inflammatory cells | Squamous epithelium | Capillary endothelium | |
Chronic periodontitis (n = 71) | ||||||
PG and TF | 48 (68%) | 19 (27%) | 14 (20%) | 11 (15%) | 4 (6%) | 53 (75%) |
TF only | 14 (20%) | 19 (27%) | 20 (28%) | 5 (7%) | 4 (6%) | 14 (20%) |
both negative | 9 (13%) | 33 (46%) | 37 (52%) | 55 (77%) | 63 (89%) | 4 (6%) |
Aggressive periodontitis (n = 11) | ||||||
PG and TF | 7 (64%) | 4 (36%) | 4 (36%) | 1 (9%) | 0 | 6 (55%) |
TF only | 1 (9%) | 1 (9%) | 1 (9%) | 0 | 0 | 2 (18%) |
both negative | 3 (27%) | 6 (55%) | 6 (55%) | 10 (91%) | 11 (100%) | 3 (27%) |
Total (n = 82) | ||||||
PG and TF | 55 (67%) | 23 (28%) | 18 (22%) | 12 (15%) | 4 (5%) | 59 (72%) |
TF only | 15 (18%) | 20 (24%) | 21 (26%) | 5 (6%) | 4 (5%) | 16 (20%) |
both negative | 12 (15%) | 39 (48%) | 43 (52%) | 65 (79%) | 74 (90%) | 7 (9%) |