Figure 2 | Scientific Reports

Figure 2

From: N-Acyldopamine induces aggresome formation without proteasome inhibition and enhances protein aggregation via p62/SQSTM1 expression

Figure 2

AcylDAs induce aggresome formation without proteasome inhibition. (A) N2a cells stably expressing GFP-vimentin were treated with 5 µM OLDA, 5 µM NADA, 10 µM anandamide (AEA), 50 µM capsaicin, or 1 µM MG132 for 12 h as indicated. G-vimentin (G-Vim, green in merged images), p62 (red), and DAPI (blue; nucleus) were visualized by fluorescence microscopy. The arrows indicate aggresomes surrounded by the vimentin cage. (B) GFP-vimentin cell lines were treated with 1 µM MG132, 5 µM OLDA, or 5 µM NADA and with or without 10 µM nocodazole (+NOC) simultaneously for 24 h as indicated. G-Vimentin (G-Vim, green), p62 (red), and S403-phospho-p62 (S403-P; blue) were visualized by confocal microscopy. Scale bars represent 5 µm.

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