Figure 6
From: Evidence for neurogenesis in the medial cortex of the leopard gecko, Eublepharis macularius
Cells generated by the ventricular zone become neurons in the cellular layer. (A) Experimental design for the 5-bromo-2′-deoxyuridine (BrdU) cell tracking experiment. BrdU was injected intraperitoneally twice daily for two days (pulse). Geckos were collected at days 0, 10, and 30 following the pulse. BrdU+ cells (hatched ellipses) were restricted to the ventricular zone at day 0 (B), had migrated into the inner plexiform layer by day 10 (C), and were observed in the cellular layer by day 30 (D). At day 0 (E), BrdU+ cells were of the ventricular zone co-localized with SOX2. By day 10 (F), BrdU+ cells within the inner plexiform layer were closely associated with GFAP+ radial processes. At day 30 (G), BrdU+ cells in the cellular layer co-localized with mature neuronal marker NeuN. Scale bar: 15 μm. cl = cellular layer, ipl = inner plexiform layer, vz = ventricular zone.
