Figure 5
From: Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes
MII oocytes contain the necessary protein machinery for NHEJ. The presence and localisation of several key enzymes within the NHEJ pathway were investigated using immunocytochemistry. Ataxia telangiectasia mutated (ATM), X-ray repair complementing defective repair in Chinese hamster cells (KU80/XRCC5), DNA-dependent protein kinase catalytic subunit (DNA PKcs) and Proliferating cell nuclear antigen (PCNA) were each identified within the MII oocyte. In addition, the active phosphorylated forms of both ATM (p-Ser1981) and DNA PKcs (p-Ser2056) were also detected within these cells. In all instances, punctate fluorescence was disseminated throughout the ooplasm. Scale bar = 80 μm.
