Figure 1
From: Adaptable, illumination patterning light sheet microscopy
Selectively patterned illumination for light sheet microscopy. (a) The coordinate system used in relation to the light sheet throughout. Illumination and detection directions shown with blue/green arrows respectively (b) Degree of patterning achievable for different illumination modes. (c) Confining illumination to ROIs within the field of view (sub-divided by grid): (i) Conventionally, the length of the ROI (single region, dx1) comes to determine the light sheet length. (ii) For dynamic or non-contiguous ROIs (multiple regions, dxn) one may deliver illumination conventionally with some inherent overexposure or selectively to the individual ROIs. (d) On-axis profile of a Gaussian beam of varying NAill along the propagation axis (widest to narrowest distribution: NA = 0.02, 0.04, 0.06, 0.08, 0.12, 0.2, 0.4). Higher NAill leads to improved confinement of light and the most equitable spread of the incoming/outgoing light to the remainder of the tissue.
