Figure 4

Illumination patterning for the 2D swept/scanned mode. (a) The fluorescent signal evolved by sweeping a focused Gaussian beam through a dye solution. (i) 42% TAG lens amplitude (ii) 61% TAG lens amplitude (98.4% of FOV within half-maximum intensity. The white line profiles show the swept beam intensity along the propagation axis. (b) Binary (digital) illumination patterns. Top: intended illumination pattern, bottom: resulting pattern seen in fluorescence (61% TAG amplitude). (c) Analog illumination patterns. First and third images: intended illumination patterns, second and fourth images: resulting pattern seen in fluorescence featuring duty cycle intensity correction (61% TAG amplitude). All scale bars: 100 μm.